Buffers and Stabilizers
Beside Substrates and Conjugates, our partner Kementec offers Buffers and Stabilizers for Immunoassays and Blotting. Kementec buffers are designed for use in colorimetric as well as in chemiluminescence based assays such as ELISA, Western Blotting and IHC. The stable and robust blocking buffers, stabilizers and diluents offered are ready-to-use and most of the buffers are ECO-TEK®.
ECO-TEK® includes:
- Free of Toxic Preservatives
- Free of Bovine Serum Albumin
- Free of Harmful Organic Solvents
Blocking and Stabilizing
Kementec's product portfolio includes traditional blocking buffers for ELISA and Blotting, as well as WellChampion® - Kementec’s new generation Plate Blocking & Protection Buffer for pre-coated plates.
Available Blocking Buffers from Kementec:
| Article Number | Product Description |
|---|---|
| KEM-4520 |
Synthetic Blocking Buffer, ELISA (Microwell Blocker) |
| KEM-4650 |
Synthetic Blocking Buffer, Blotting (Membrane Blocker, Protein Free) |
| KEM-4900A |
WellChampion® Microwell Plate Blocker/Stabilizer |
Diluents and Stabilizers
Kementec offers ready-to-use sample diluents and robust stabilizers that demonstrate superior stabilization of proteins conjugated to Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). These stabilizers prevent loss of enzyme activity and maintain the proteins molecular conformation.
Choosing the right buffer
The following table will guide you to the optimal choice of Kementec's different solutions.
D: Diluent; S: Stabilizer, B: Blocker
| Buffer | Standards & Controls | Samples | HRP-conjugates | AP-conjugates | Antibodies |
|---|---|---|---|---|---|
|
HRP-StabilPLUS* (KEM-4530) |
D/S | ||||
|
AP-StabilPLUS (KEM-4540) |
D/S | ||||
|
UNI-StabilPLUS (KEM-5230) |
D/S | D/S | |||
|
Antibody Enhancer* (KEM-4660) |
D/S | D/S | D/S | ||
|
Sample X-tra (KEM-5350) |
D | D | |||
|
Effect Diluent* |
D | D | D | D |
* Not Animal-free, cannot be used with antibodies against rabbit immunoglobulin