Substrates for Immunoassays
Our partner Kementec’s portfolio of colorimetric substrates includes soluble Horseradish Peroxidase (HRP) substrates (TMB) and soluble Alkaline Phosphatase (AP) substrates (pNPP) and precipitating substrates enabling different substrate formulations for ELISA and for membrane assays.
Kementec's substrates are ECO-TEK, making them REACH, Annex XVII0 compliant and easy to transport with less restrictions. ECO-TEK includes:
- Free of Toxic Preservatives
- Free of Harmful Organic Solvents – No NMP
- Free of Hazardous Stop Solutions
The substrates moreover offer outstanding sensitivity, stop-stability and shelf life stability, and negligible lot-to-lot variation. Offering a shelf life up to 4 years of the ready-to-use substrates enables Kementec products to meet all the requirements of assay manufacturers.
Soluble Horseradish Peroxidase (HRP) Substrates
In a soluble chromogenic HRP assay setup, like an ELISA, the preferred substrate is TMB (3, 3', 5, 5'-tetramethylbenzidine). When developing immunoassays, choosing the right TMB is a vital step for optimizing the assay. Kementec offers a range of TMBs with different kinetics and sensitivities, enabling assay developers to choose the right TMB for their assay. All the TMBs are ECO-TEK.
TMB substrates are also available as unique color-coded products for traceable pipetting. The pink indicator makes manual pipetting traceable, while in automatic analyzers it offers a convenient tracking system for reagent supply. The color disappears after adding the recommended stop solution and does not interfere with the assay.
Corresponding diluents are available for TMB substrates in order to achieve a specific activity level.
Available TMB ELISA HRP Substrates from Kementec:
| Article Number | Product Description |
|---|---|
|
TMB ONE (& prestained) ELISA HRP Substrate |
|
| KEM-5320 |
TMB EASE ELISA HRP Substrate |
|
TMB PLUS2® (& prestained) ELISA HRP Substrate |
|
| KEM-5280 |
TMB X-tnd ELISA HRP Substrate |
|
TMB X-tra (& prestained) ELISA HRP Substrate |
Soluble Alkaline Phosphatase (AP) Substrates
In a soluble chromogenic Alkaline Phosphatase (AP) assay setup, like an ELISA, the preferred substrate is pNPP (p-nitrophenylphosphate). Kementec offers two different pNPP solutions, which are mono-component and stable for up to 36 months.
pNPP liquid ready-to-use substrate is highly active. The substrate gives superior results due to its sensitivity, allowing very low detection limits. pNPP ONE liquid ready-to-use substrate is incredibly stable. PNPP ONE shows superior background characteristics due to its high temperature robustness. Both pNPPs are ECO-TEK.
Available pNPP ELISA AP Substrates from Kementec:
| Article Number | Product Description |
|---|---|
| KEM-4400 |
pNPP ONE ELISA AP Substrate |
| KEM-4401 | pNPP ELISA AP Substrate |
Precipitating Substrates
Kementec offers superior precipitating substrates for AP (BCIP/NBT) and HRP (TMB). The substrates are ready-to-use, robust and highly sensitive for applications like membrane blotting. The intense colored precipitates are incredibly stable and resist fading over time. The TMB Blotting substrates are ECO-TEK.
Available Precipitating Substrates from Kementec:
| Article Number | Product Description |
|---|---|
| KEM-4410 |
BCIP/NBT Precipitating AP Substrate |
| KEM-4450 |
TMB Blotting Precipitating HRP Substrate |
| KEM-4600 |
TMB-D Blotting Precipitating HRP Substrate |
The range is complemented by matching Buffers and Stabilizers as well as Labels for Conjugation.