New Anti-Influenza A/B Antibodies

Rabbit monoclonal anti-Influenza antibodies from GeneTex

New recombinant Anti-Influenza A/B Antbodies from GeneTex

 

Influenza A and B viruses are two viruses that have caused seasonal influenza, epidemics, and even pandemic for Influenza A.

GeneTex has released three clones each for Influenza A and B Nucleoproteins, the nucleoprotein is conserved in different variants therefore a suitable target for detecting the virus antigen.

 

• The antibodies can work as ELISA pairs and showed a low picomolar range sensitivity
• At least sixteen FLUAV strains and nine FLUBV strains can be detected by ELISA and western blot, including H1N1 and H3N2 subtypes

 

Antibody	Method	Clonality	Ordering No
Influenza A virus Nucleoprotein antibody [HL1103]	ELISA, Sandwich ELISA	Rabbit Monoclonal	GTX636318
Influenza A virus Nucleoprotein antibody [HL1078]	ELISA, Sandwich ELISA	Rabbit Monoclonal	GTX636199
Influenza A virus Nucleoprotein antibody [HL1089]	ELISA, Sandwich ELISA	Rabbit Monoclonal	GTX636247
Influenza B virus Nucleoprotein antibody [HL1068]	WB, ELISA, Sandwich ELISA	Rabbit Monoclonal	GTX636099
Influenza B virus Nucleoprotein antibody [HL1073]	WB, ELISA, Sandwich ELISA	Rabbit Monoclonal	GTX636194
Influenza B virus Nucleoprotein antibody [HL1069]	WB, ELISA, Sandwich ELISA	Rabbit Monoclonal	GTX636100

 

 

Recombinant Nucleoprotein antibodies are validated for sandwich ELISA.

GeneTex’s Nucleoprotein antibodies have been tested for sandwich ELISA using recombinant nucleoproteins, and showed a low picomolar range sensitivity. The Flu A pairs can detect at least 16 strains of virus lysates, and the Flu B pairs have been tested on 7 strains of virus lysates. The Influenza A antibodies showed no cross reactivity to Influenza B, and the Influenza B antibodies showed no cross reactivity to Influenza A.

Figure 1. Detection of FLUAV and FLUBV NPs in sELISA assays. Recombinant NPs derived from different strains of influenza A virus and influenza B virus were assayed using (A) FLUAV NP antibodies GTX636318 and GTX636247 (as a pair), or (B) FLUBV NP antibodies GTX636099 and GTX636100 (as a pair).

Figure 2. Analysis of influenza virus strains. Sandwich ELISA detects NPs in lysates from cells infected with different strains of (A) influenza A virus subtype H1N1 or (B) subtype H3N2 using FLUAV NP antibodies GTX636318 and GTX636247 (as a pair), or (C) influenza B virus using FLUBV NP antibodies GTX636099 and GTX636100 (as a pair). (D) The FLUAV NP antibody GTX636199 recognizes NPs of various FLUAV subtypes in infected-cell lysates by western blot.

 

 

Table 1. FLUAV strain NPs detected by GeneTex antibodies GTX636318 and GTX636247

 

A/Puerto Rico/8/34 (H1N1)	A/New Caledonia/20/99 (H1N1)
A/Singapore/63/04 (H1N1)	A/Taiwan/42/06 (H1N1)
A/Brisbane/59/07 (H1N1)	A/New York/02/09 (H1N1)
A/Mexico/4108/09 (H1N1)	A/Canada/6294/09 (H1N1)
A/California/07/09 (H1N1)	A/New York/01/09 (H1N1)
A/New York/03/09 (H1N1)	A/Texas/50/12 (H3N2)
A/Hong Kong/8/68 (H3N2)	A/Wisconsin/67/05 (H3N2)
A/Brisbane/10/07 (H3N2)	A/Perth/16/09 (H3N2)

 

Sensitivity of the different Antibodies against FLUAV strains compared to other commercially available antibodies

 

Table 2. FLUBV strain NPs detected by GeneTex antibodies GTX636099 and GTX636100

 

B/Victoria/02/87	B/Panama/45/90
B/Florida/07/04	B/Sydney/3/04
B/Florida/02/06	B/Florida/04/06
B/Brisbane/33/08	B/Wisconsin/1/10
B/Massachusetts/2/12

Sensitivity of the different Antibodies against FLUBV strains compared to other commercially available antibodies