IHC-plus™ Antibodies from LSBio

...because seeing is believing!

LSBio (LifeSpan Biosciences) offers a catalog of 130,000 antibodies that have been tested for use in a variety of research applications, including immunohistochemistry, ELISA, Western blot, and flow cytometry. 35,000 of these antibodies have been tested and received validation for use in IHC. Of these, 23,000 antibodies have been extensively tested in LSBio's Seattle laboratory and awarded IHC-plus™ brand validation. IHC-plus™ antibodies from LSBio have been identified as the best reagents for use in FFPE-IHC.

LSBio IHC-plus Immunohistochemistry Validation

LSBio's IHC-plus™ antibodies have been tested and identified as being optimal for use in immunohistochemistry (IHC) against formalin-fixed paraffin-embedded (FFPE) human tissues

LSBio's IHC protocol has been developed over the past 15 years as the most optimal method of immunolabeling FFPE tissues, the most common fixation method used by pathology labs worldwide.

> Each antibody is tested at multiple concentrations on more than 20 normal human tissue types, and when appropriate, multiple normal brain regions and/or cancer types.

> A LifeSpan pathologist with extensive experience evaluating IHC analyzes the localization profile of each antibody, identifying positive and negative cell types, signal strength, subcellular and extracellular staining, and staining artifacts.

> This information is compared with all published expression and localization data available for the protein. This enables LSBio to evaluate how each antibody behaves in IHC, including its specificity to the target protein, its sensitivity of detection, and any non-specific staining characteristics that it may display.

In order to be selected as an IHC-plus™ brand antibody, antibodies must have a close correlation to the published literature, be high affinity, display minimal staining artifacts, and have a high signal-to-noise ratio, such that its specific staining is considerably higher than its level of nonspecific background staining.

See and believe: LSBio offers over 23,000 IHC-plus validated antibodies for a comprehensive range of targets and research areas.