QuickSwitch™ Tetramer Platform

NEW: SARS-CoV-2 Peptide Bundles

Functional screening of peptides for MHC class I binding is essential for vaccine development and immune system monitoring. A fast, high-throughput and user-friendly assay system with potential for clinical immune monitoring is needed today more than ever.

MBL International offers QuickSwitch™ kit for you to create your own custom MHC tetramer.  This kit is supplied with a tetramer bound with an irrelevant peptide.  After adding a peptide exchange factor and your peptide of interest, peptide exchange will occur if there is binding affinity of the peptide with the MHC molecule.  By using the QUANT kit, you can quantify the amount of exchange that occurred, thereby gathering binding affinity information for the MHC/peptide complex.  This kit can be used to create custom tetramers, or for screening peptides, all in one day.  QuickSwitch™ is a 90-min-assay system that allows discrimination of MHC binding from non-binding peptides.

This is particularly essential for the screening of immunogenic peptides from infectious agents or cancer neoantigens.  Tetramers resulting from peptide exchange with selected peptides can then be used for immune monitoring.  Functional screening of peptides for MHC class I binding is essential for vaccine design and immune monitoring.

Advantages of the QuickSwitch™ Tetramer platform:

• Validation of in silico pre-selected peptides for their MHC-binding properties
• Generation of new specific tetramers for immune monitoring
• Functional stability studies for MHC-binding peptides
• Epitope comparison for epitope mapping of better binding peptides

MBL QuickSwitch™: Peptide exchange, quantification, cell staining and flow cytometric analysis within one day!

 

NEW: QuickSwitch™ and SARS-CoV-2 peptide bundles

The proteome of the new coronavirus SARS-CoV-2 is known, potential B- and T-cell epitopes have been identified and published, e.g. by Grifoni et al.  Peptide screening with the QuickSwitch™ platform allows to identify and validate the binding of predicted peptide sequences of COVID-19 virus proteins in the most common alleles: HLA-A2, A11, A24, A3 and HLA-DR1, DR4, DR15. 

MBLI has researched and tested SARS-CoV-2 peptide sequences of interest to assess peptide-MHC binding using QuickSwitch™ kits.  These peptides are bundled with their respective QuickSwitch ™ allele. By purchasing a bundle, you can create up to 8 custom tetramers in 1 day- saving you significant time and money.  Because the peptide/MHC binding was already tested, you don’t have to do any preliminary testing, you can quickly jump into T cell staining assays with your new custom tetramers specific for SARS-CoV-2.

 

More information:

 

VIDEO: MHC Tetramers - an introduction