Recombinant Protein Expression

MoBiTec Cloning and Expression Vectors

Superior recombinant protein expression platforms accelerate basic research on protein structure and function. They can also be used for large-scale production of enzymes and biotherapeutics. Choosing the right protein expression system for your specific application is key to success.

Under the brand MoBiTec we offer a wide range of unique bacterial and yeast protein expression systems to suit your research needs. Our expression tools are easy to use, designed for high protein yields and fast results.

 

Huge Selection of Cloning and Expression Vectors

MoBiTec is leading in the development of unique and cutting edge vector systems for recombinant protein expression. We offer a huge selection of easy-to-handle tools for efficient gene expression in different microorganisms like Bacillus megaterium, Bacillus subtilis, Lactococcus lactis, Escherichia coli, and yeast.

In addition to the vectors, several useful host strains are available, including ready-to-use protoplasts for Bacillus megaterium which is difficult to transform.

 

Bacillus megaterium Expression Systems

The Gram-positive bacterium Bacillus megaterium does not possess alkaline proteases and is known for stable replication and maintenance of plasmids. Furthermore, the bacterium is able to secrete proteins into the growth medium.

Features

• Versatile protein production (intra- & extracellular)
• Inducible & constitutive expression systems
• Endotoxin-free
• Simple purification with different tags
• Protoplasts specifically optimized for transformation

> Bacillus megaterium expression vectors

> Bacillus megaterium protoplasts

 

Bacillus subtilis Expression Systems

With its ability to secrete proteins directly into culture media, amenability to medium- and large-scale fermentation, no significant bias in codon usage, and designation by the U.S. FDA as an organism that is Generally Regarded As Safe (GRAS), it‘s not surprising that the majority of industrially-produced enzymes are expressed in Bacillus subtilis.

Features

• Tightly controlled gene expression / suitable for metabolic engineering
• Avoid expensive inducers (e.g., IPTG)
• Strain with reduced protease activity available
• Endotoxin-free
• Prevent protein aggregation
• E. coli / B. subtilis shuttle vectors with different tags
• Different constitutive expression systems and vectors for high-level extracellular protein production

> Bacillus subtilis expression vectors

> Bacillus subtilis strains

 

Lactococcus lactis Expression Systems

Lactococcus lactis has a long history of safe usage in food production. Several expression systems, notably the NICE system, have been developed for this organism. The NICE system allows for tightly controlled expression using the food grade inducer peptide nisin.

Features

• Stable, high-yield protein production; easy-to-handle
• Simple fermentation, scale-up & downstream processing
• Safe platform for production of biotherapeutics and diet components
• Intracellular production or secretion
• Inducible and constitutive expression systems
• Endotoxin-free, no inclusion bodies, reduced protease activity
• Expression of membrane proteins
• Suitable also for other lactic acid bacteria

> Lactococcus lactis expression vectors

> Lactococcus lactis strains

 

Gene Expression in Pichia pastoris: pPICHOLI vectors

The pPICHOLI Shuttle vector system is a dual expression system with many advantages: Expression with E. coli is simple to handle and allows a cost-effective and high-level production of heterologous proteins. Further, the pPICHOLI vector system is a powerful eukaryotic tool and can be used for expression with P. pastoris. 

Features

• Efficient, cost-effective, and time-saving protein production in either E. coli or P. pastoris
• Gene products toxic to E. coli may be easily expressed in P. pastoris
• No further cloning step needed: switch from E. coli expression to P. pastoris expression without changing the vector.
• Expression with P. pastoris may help to increase the protein yield by avoiding aggregation, denaturation, or formation of inclusion bodies of your protein of interest.
• Vectors can be used for in vitro transcription/translation of cloned genes
• Easy, fast, and reproducible purification of recombinant proteins via metal chelate affinity chromatography (His-tag)
• Detection of recombinant proteins in immunoblotting experiments by commercially available antibodies
• Biotinylation sequence provided by pPICHOLI-C allows rapid capture and screening assays
• Production of functional target proteins
• Synthesis of proteins for crystallization and NMR analysis
• Expression of proteins in high-throughput systems

> pPICHOLI Shuttle vector system