Electron microscopy

Molecular Dimensions ANTcryo™ Cryo-EM Grids

Our partner Molecular Dimensions (a Calibre Scientific company) is a world leading supplier of modern screens, reagents, other consumables and instrumentation for protein structure determination by X-ray crystallography. Molecular Dimensions offers the most innovative range of products from the latest developments in structural biology research.

 

With ANTcryo™ holey support film (Amorphous Nickel Titanium alloy Grid), Molecular Dimensions presents a new and sophisticated solution for cryo-EM singleparticle sample preparation. Users of the ANTcryo™ Grid have published more than 20 papers on high-index Journal in last 3 years, 6 of which were published on CNS. ANTcryo™ has extraordinary benefits and is widely recognised by cryo-EM community.

 

Advantages of ANTcryo™ Grids

Improving the resolution of reconstruction
ANTcryo™ Grids offer increased resolution compared to carbon grids due to their superior conductivity, resulting in reduced electron charging effects and less beam-induced motion (BIM) during cryo-EM imaging. This improvement enables the achievement of higher-resolution structures, with user experiments demonstrating an improvement of more than 0.2 Å in resolution.

Improving the rate of sample preparation
ANTcryo™ support film exhibits an adsorption force nearly 20 times lower than traditional carbon film, thereby enhancing the particle inlet rate and reducing particle aggregation within the pore. This improvement significantly enhances the success rate of sample preparation by improving particle dispersion.

Improving the efficiency of data collection
With the contribution of higher particle density, users need much less EM time for data collection for the same number of particles. Similar to carbon films, ANTcryo™ Grids are made of amorphous material that is suitable for coma alignment. Unlike gold films, ANTA film’s amorphous structure allows routine TEM alignment like carbon films (defocus estimation, stigmatism adjustment, coma-free alignment). This eliminates challenges associated with gold’s polycrystalline nature, simplifying cryo-EM workflows and saving valuable research time.

Fig: Cryo-EM photo of apoferritin. Compared with traditional carbon membrane, ANTcryoTM support membrane has higher particle density, less overlapping of particle dispersion, and shorter data collection time; the particle boundary is clear and the resolution obtained much higher.

 

Available ANTcryo™ Grids

Art. N°	Product description
MDL-MDL-M01	ANTcryo ANTA Cryo-EM Grids (Au300-R1.2/1.3), 50 Grids
MDL-MDL-M02	ANTcryo ANTA Cryo-EM Grids (Cu300-R1.2/1.3), 50 Grids
MDL-MDL-M03	ANTcryo ANTA Cryo-EM Grids (Au400-R1.2/1.3), 50 Grids
MDL-MDL-M04	ANTcryo ANTA Cryo-EM Grids (Au300-R2.0/1.0), 50 Grids