iLite® Cell-Based Solutions

Simple and reproducible cell-based reporter gene assays from Svar

Svar is a Swedish life science company that invent, develop and apply the best assay technology for drug development and clinical diagnostics with the goal to deliver new solutions tailored to customer requirements. Svar establish practical platforms for clinical routine testing, work to secure relevant competences by sharing best practices and knowledge and their partnerships enable them to deliver flexible solutions depending on specific needs.

The iLite® cell-based solutions from Svar are based on the iLite technology, a cell-based assay system with dual reporter gene readout. They offer the ease of use and robustness of a Ligand Binding Assay and can be developed for virtually any pharmaceutical target and allows an easy, rapid and accurate test format for measurement and quantification of drug activity and immunogenicity.

Cell based assays are defined as any assay that takes place within a living cell. Because this definition is so general, there are thousands of different cell based assays. Reporter-gene assays are a specific kind of cell-based assay with an engineered system that incorporates a regulatory elements that control a given gene and that use an easily detectable readout like luciferase.

Assay Ready Cells - As easy as 1-2-3

Save time and money by not having to culture your own cells

The iLite® cell-based solutions are supplied as cryopreserved assay ready cells, that are harvested and frozen at the same time, which means a higher reproducibility and overall superior performance compared to cells in culture can be obtained. Not the mention the time and cost you can reduce but not having to culture your cells!

1. Thaw

Start by thawing your assay-ready cells. All cells were harvested and frozen at the same stage which ensures excellent reproducibility and performance.

2. Plate

Next, plate your cells and add your sample. The cells contain a secondary reporter gene that can be used as a powerful tool for normalizing results and to compensate for differences in cell numbers.

3. Incubate & Read

Finally, incubate and read your assay. The combination of excellent target specificity and a high signal-to-noise ratio ensures a biologically relevant functional assay.

iLite® FUNCTIONAL BIOASSAYS

The iLite Functional Bioassay portfolio is based on a reporter gene system and offers a convenient and powerful way of measuring and quantifying drug potency, detecting neutralizing antibodies (NAbs) and assessing immunogenicity.

Reporter gene cell lines allow for detection of signal which is directly proportional to the induced function of your target compound.
A reporter gene is inserted in the cell under an inducible promotor and the gene is expressed to give a quantifiable readout. The iLite technology utilizes the existing intra cellular system, thereby maintaining the biological relevance of the assay. A unique “lock and key” function has been developed in the iLite cell lines which amplifies the signal and makes it more specific, resulting in a more sensitive cell and a larger dynamic range.
The iLite Functional Bioassay portfolio consists of cell lines provided in an “assay-ready” format for a rapid and convenient workflow. The assay ready format also reduces assay variability, optimizing their use in screening, characterization, stability and potency studies. iLite cell lines can be tailored to virtually any biopharmaceutical target.

iLite® FUNCTIONAL BIOASSAY PORTFOLIO:

Complement Products

iLite® C5a [EUD-BM4075]

Cytokine responsive

iLite® RANKL [EUD-BM4052]
iLite® TNF-alpha [EUD-BM3044]
iLite® Type I IFN [EUD-BM3049]
iLite® Type I IFN FAST [EUD-BM4049]

Hormone responsive

iLite® hCG [EUD-BM4080]
iLite® Insulin [EUD-BM3060]

Interleukine responsive

iLite® IL-2 [EUD-BM4002]
iLite® IL-6 [EUD-BM4061]
iLite® IL-12 [EUD-BM4012]
iLite® IL-23 [EUD-BM4023]

Growth Factors

iLite® VEGF [EUD-BM4021]
iLite® FGF-21 [EUD-BM3071]
iLite® GM-CSF [EUD-BM4050]
iLite® G-CSF [EUD-BM4055]

Toll-Like Receptors