Vector Labs Mounting Media

VECTASHIELD for IF and VectaMount for IHC

Vector Laboratories is the market leader in labeling and detection reagents for immunohistochemistry, immunofluorescence, and bioconjugation with 40 years of experience. Vector Labs was first company to commercialize avidin-biotin enzyme complex kits (VECTASTAIN® ABC kits) for immunohistochemistry (IHC) and antifade mounting media (VECTASHIELD® Mounting Media) for immunofluorescence (IF).

Vector Laboratories offers you a comprehensive range of kits and reagents for every step in your IHC workflow or IF workflow.

 

VectaMount® Mounting Media for Immunohistochemistry (IHC)

VectaMount AQ Mounting Medium (VEC-H-5501) is a hardsetting mounting medium developed for use with enzymatic substrates, such as AEC, ImmPACT® AEC, and ImmPACT AMEC Red, whose reaction products are soluble in alcohol or other organic solvents. In applications where aqueous mounting is preferred, VectaMount AQ is suitable for use with other substrates such as DAB, Vector® SG, BCIP/NBT, Vector Red, ImmPACT Vector Red, Vector Blue, ImmPACT DAB, ImmPACT DAB EqV, and ImmPACT SG.

Features: For aqueous mounting Compatible with many horseradish peroxidase and alkaline phosphatase substrates

 

 

VectaMount® Express is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following immunohistochemistry (IHC) staining. The novel formulation is engineered to enable mounting directly following staining, saving time by eliminating the need for extensive ethanol and clearing washes prior to coverslipping. Just stain your slides as per your usual workflow, briefly wash in isopropyl alcohol, and coverslip.

Features: fast non-aqueous mounting Eliminates the need for extensive ethanol washes and xylene Rapid drying formula

 

 

VECTASHIELD® Antifade Mounting Media for Immunofluorescence

VECTASHIELD® next generation mounting medium for immunofluorescence (IF) stainings is offered in setting and non-setting formulations with a choice of counterstain or no counterstain. All Vectashield Mounting Media share an ideal refractive index and easy application, and don't need sealing of coverslips.

VECTASHIELD TOPSELLER:

VECTASHIELD Product	Setting Formulation	Counterstain	Refractive Index	go to Shop
VEC-H-1000	non-hardening	none	1.45	VEC-H-1000
VEC-H-1200	non-hardening	DAPI	1,45	VEC-H-1200
VEC-H-1400	hardening	none	1.45	VEC-H-1400
VEC-H-1500	hardening	DAPI	1.45	VEC-H-1500

 

VECTASHIELD PLUS Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD PLUS Mounting Medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes. The original VECTASHIELD PLUS Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 2-8 °C, protected from light.

Features: Ideal refractive index Ready to use No warming necessary Can be stored without sealing for long term analysis Easy to use

 

 

VECTASHIELD Vibrance Antifade Mounting Media are completely new formulations of curing (hard setting) antifade mounting media for immunofluorescence applications. These newest additions to our VECTASHIELD portfolio were developed with customer feedback to improve on parameters such as ease of use and retention of specific staining intensity over time. VECTASHIELD Vibrance Antifade Mounting Media are new tools to help investigators see more and do more with each experiment.

Features: Superior antifade/anti-photobleaching properties across the spectrum Compatibility with commonly used fluorophores View sections one hour after mounting No tone or autofluorescent background, even after curing Room temperature storage of slides with extended archiving time Minimal bubble formation, even after several weeks storage Curing formulations with choice of counterstain (DAPI) or no counterstain  12 month product expiration date

 

 

Why should I use a Mounting Medium for my fluorescent staining?

 

The Mounting medium is the "connective tissue" between your specimen and the glass slide and coverslip and provides protection to your sample. In addition, the mounting medium may help with other issues that you might encounter with your staining:

• preventing cells from drying out
• protecting your immunofluorescent signals from fading or photobleaching
• Keep the sample safe and preserve it´s properties for short or long-term storage
• To improve the optical clarity of samples for better viewing under the microscope