The original mouse IgG2a version of this antibody was raised against sucrose-gradient purified Ectromelia virus (ECTV) Strain Munich 1.
Clonality:
Monoclonal
Clone Designation:
[VACV-5B1]
Isotype:
IgG
Buffer:
PBS with 0.02% Proclin 300.
Source:
Mouse
Target:
Orthopoxviruses (incl Ectromelia & Monkeypox)
ELISA using VACV-5B1 (Ab02220), recombinant MPXV A29 Protein and recombinant MPXV L1R Protein. Binding curves of the rabbit IgG chimeric version of the Anti-Orthopoxviruses antibody VACV-5B1 (Ab02220-23.0; blue line) and isotype control (Ab00102-23.0, anti-fluorescein; orange line) to an ELISA plate coated with recombinant MPXV A29 Protein and recombinant MPXV L1R Protein at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Ab02220 antibody. For signal detection, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.
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