It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and
200µg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity:
Protein A/G
Form:
Purified
Formula:
200 µg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Target:
IVL
Application Dilute:
Does not react with mouse.Flow Cytometry (0.5-1µg/million cells), ,Immunofluorescence (1-2µg/ml), ,Immunohistology (Formalin-fixed) (0.1-0.2µg/ml for 30 min at RT),(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV
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