HotTaq DNA polymerase catalyzes the 5-3 synthesis of DNA. The enzyme has been proven not to have the 3 - 5exonuclease activity. Before the first PCR step, the HotTaq DNA polymerase should be activated by 15 minute incubation at 95-97C.
Concentration:
10 units/uL
Buffer:
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
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