In addition to 5-3 DNA polymerase activity, it also possesses 3-5 exonuclease (proofreading) activity. Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, and is even up to ten-fold more accurate than norm
Concentration:
5 units/uL
Buffer:
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
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