Alkaline Phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. Humans and most other mammals contain the following alkaline phosphatase isozymes: ALPI - int
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Application Notes:
Anti-Alkaline Phosphatase peroxidase has been tested by dot blot and is functional in ELISA, Western blot, and Immunohistochemical assays. Optimization should be reached by researcher.
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