Pepsin is one of three principal protein-degrading, or proteolytic, enzymes in the digestive system, the other two being chymotrypsin and trypsin. Pepsin is commonly used in the preparation produce either an Fab or an F(ab)2 fragment from antibodies.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immun
Application Notes:
Anti-Pepsin peroxidase has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided.
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