This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).
Recognizes the 51 amino acid (6 kDa) insulin polypeptide composed of A and B chains. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insul
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This protein A purified mouse monoclonal antibody reacts specifically with insulin from human and swine sources. Cross reactivity with insulin from mouse and rat does not occur. Cross reactivity with insulin from other sources has not been determined.
Application Notes:
Mouse Anti-Insulin Antibody has been tested by ELISA and dot blot and is suitable for immunohistochemistry, immunoblotting and immunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or b-cells from islets of Langerhans can be used
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