GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.
GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5-AGATAG-3 on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have bee
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography. A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93% homology. Cross-reactivity with GATA4 from other sources has not been d
Application Notes:
Anti-GATA4 antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysat
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