Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.
Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This antibody is directed against human Ubiquitin Activating Enzyme E1 protein. The product was protein A purified from monospecific antiserum followed by further purification to remove the GST tag. A BLAST analysis was used to suggest that this antibody
Application Notes:
This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kDa in size corresponding to UBE1 by western blotting in the app
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