This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease.
TEV protease, encoded by the Tobacco Etch Virus (TEV), is a 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the virus (TEV). It is widely used for cleaving fusion proteins because of its sequence specificity. It recognizes a l
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. Assay by western blot showed no reactivity to MBP.
Application Notes:
This protein A purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate c
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