This Anti-PhosphoTyrosine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.
Phosphotyrosine is the tyrosine amino acid that has been phosphorylated and is considered one of the integral steps in signal transduction and general enzyme activity. Tyrosine is phosphorylated through protein kinases and through phosphorylation, phosph
Clonality:
Polyclonal
Concentration:
250 µg/mL
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-PhosphoTyrosine (pY) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit
Application Notes:
Anti-PhosphoTyrosine (pY) Antibody is tested for use in ELISA, western blotting, immunohistochemistry-P, immunoprecipitation, and Non-radioactive Tyrosine kinase assays. Anti-PhosphoTyrosine (pY) Antibody reacts specifically with phosphotyrosine and show
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