This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 175-200 of mouse MDM2.
MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the murine double minute 2 gene (mdm2). The MDM2 gene enhances the
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This affinity-purified antibody is directed against the phosphorylated form of mouse MDM2 protein at the pS185 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the
Application Notes:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 102 kDa in size corresponding to phosphorylated MDM2 protein by weste
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