This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 110-135 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consis
RFA2 binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). It stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This affinity purified antibody is directed against yeast RFA2. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resu
Application Notes:
This affinity-purified antibody was tested for use in western blotting against RFA2 containing cell lysates and by ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.
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