This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 350-375 of the Human Rad 52 protein.
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human RAD52 protein.
Application Notes:
This affinity purified antibody has been tested by WB and ELISA. Anti-RAD52 is useful in western blotting using HeLa nuclear extracts. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibo
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