PsbA | D1 protein of PSII positive control/quantitation standard
Biozol Catalog Number:
AGR-AS01-016S
Supplier Catalog Number:
AS01-016S
Alternative Catalog Number:
AGR-AS01-016S
Manufacturer:
Agrisera
Category:
Proteine/Peptide
Application:
WB
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination
Molecular Weight:
The standard has an actual MW of 41,5 kDa, The presence of a His6 tag causes it to run ~1,7 kDa higher on the gel than the native protein, Note that in most systems, PsbA migrates with an apparent MW of between 30 and 35 kDa,
Form:
Lyophilized in glycerol.
Antibody Type:
Secondary Antibody
Application Dilute:
Standard curve: 3 loads are recommended (0.5, 2 and 4µl).For most applications a sample load of 0.2 µg of chlorophyll will give a PsbA signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection systems.Non-
Application Notes:
Concentration: after adding 95 l of sterile milliQ water final concentration of the standard is 0.25 pmoles/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red
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