Anti-RbcL | Rubisco large subunit, form I (chicken), Polyclonal

Catalog Number: AGR-AS01-017
Article Name: Anti-RbcL | Rubisco large subunit, form I (chicken), Polyclonal
Biozol Catalog Number: AGR-AS01-017
Supplier Catalog Number: AS01-017
Alternative Catalog Number: AGR-AS01-017
Manufacturer: Agrisera
Host: Gallus
Category: Antikörper
Application: IGS, IL, WB
Species Reactivity: A. thaliana, Plant
Immunogen: KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants
Clonality: Polyclonal
Molecular Weight: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
NCBI: 844754
UniProt: O03042
Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Form: Liquid
Antibody Type: Polyclonal Antibody
Application Dilute: (IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 g of total cellular protein, (WB)
Application Notes: This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-79