RbcL | Rubisco positive control/quantitation standard
Biozol Catalog Number:
AGR-AS01-017S
Supplier Catalog Number:
AS01-017S
Alternative Catalog Number:
AGR-AS01-017S
Manufacturer:
Agrisera
Category:
Sonstiges
Application:
WB
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants
Molecular Weight:
52.7 kDa
Form:
Lyophilized in glycerol.
Antibody Type:
Secondary Antibody
Application Dilute:
Standard curve: three protein standard loads are recommended.For most applications a sample load of 0.2 µg of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection sys
Application Notes:
Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Pheno
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