34 kDa (larger than a native protein due to the addition of His-tag)
Form:
Lyophilized
Application Dilute:
Standard curve: 3 loads are recommended (0.5, 2 and 4 µl).For most applications a sample load of 0.2 µg of chlorophyll will give a NifH signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection systems. Th
Application Notes:
Concentration: after adding 90 l of milliQ water final concentration of this standard is 0.15 pmoles/ul and this reagent is ready to use and load on a gel.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA
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