ATP synthase is the universal enzyme that stnthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
Molecular Weight:
in most gel systems AtpB migrates around 50-54 kDa
Form:
Lyophilized, in glycerol.
Antibody Type:
Secondary Antibody
Application Dilute:
Standard curve: 3 loads are recommended (0.5, 2 and 4µl).For most applications a sample load of 0.2µg of chlorophyll will give a AtpB signal in this range.Positive control: load per well: a 2µl load is optimal for most chemiluminescent detection systems.
Application Notes:
Concentration: after adding 90 l of dest. water final concentration of the standard is 0.27 pmol/ l.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM,
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