Anti-RFA | Bakers yeast replication factor A, Polyclonal

Product Overview

• Article Name: Anti-RFA | Bakers yeast replication factor A, Polyclonal
• Biozol Catalog Number: AGR-AS07-214
• Supplier Catalog Number: AS07-214
• Alternative Catalog Number: AGR-AS07-214
• Manufacturer: Agrisera
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IP, WB
• Species Reactivity: Yeast
• Immunogen: RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa), overexpressed in E.coli and purified by chromatography, no affinity tags were added to any of three subunits

Product Description

Saccharomyces cerevisiaereplication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA rep

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 70 + 30 + 14 kDa
• NCBI: 851266
• UniProt: P22336
• Purity: Serum
• Form: Lyophilized
• Antibody Type: Polyclonal Antibody

Application Notes

• Application Dilute: ChIP, 1 : 20 000 (WB)
• Application Notes: Antibody was also successfully used in ChIP application Holstein et al. (2014).Load of 1 ng of the protein will allow to visualize two subunits of RPA, while load of 5 ng will allow to visualize all three subunits in Western blot technique.