KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456 , TAIR: At4g30190),
Clonality:
Polyclonal
Molecular Weight:
90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
Antigen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Form:
Liquid
Application Dilute:
1 : 100 (IL), 1 : 1000-1 : 5000 (WB)
Application Notes:
VERY IMPORTANT: Please, do not heat up your samples above 70 C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any agg
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