GlnA | glutamine synthetase positive control/quantitation standard
Biozol Catalog Number:
AGR-AS09-018S
Supplier Catalog Number:
AS09-018S
Alternative Catalog Number:
AGR-AS09-018S
Manufacturer:
Agrisera
Category:
Sonstiges
Application:
WB
Glutamine synthetase (GlnA) is the key enzyme in the incorporation of mineral nitrogen into glutamine.This product is a recombinant GlnA protein standard, source Synechocystis strain PCC 6803.
Molecular Weight:
in most gel systems GlnA migrates around 53 kDa
Form:
Lyophilized
Antibody Type:
Secondary Antibody
Application Dilute:
Standard curve: 3 loads are recommended (0.5, 2 and 4µl).For most applications a sample load of 0.2µg of chlorophyll will give a GlnA signal in this range.Positive control: a 2µl load per well is optimal for most chemiluminescent detection systems. This
Application Notes:
Concentration: after adding 225 l of milliQ water final concentration of the standard is 0.20 pmol/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM,
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