PsbD | D2 protein of PSII positive control/quantitation standard
Biozol Catalog Number:
AGR-AS09-146S
Supplier Catalog Number:
AS09-146S
Alternative Catalog Number:
AGR-AS09-146S
Manufacturer:
Agrisera
Category:
Proteine/Peptide
Application:
WB
D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step i
Molecular Weight:
In most gel systems PsbD migrates around 28-30 kDa
Form:
Lyophilized in glycerol
Antibody Type:
Secondary Antibody
Application Dilute:
Standard curve: 3 loads are recommended (0.5, 2 and 4µl).For most applications a sample load of 0.2 µg of chlorophyll will give a PsbD signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection systems. Thi
Application Notes:
Concentration: after adding 225 l of milliQ water final concentration of the standard is 0.25 pmoles/ulProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 m
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