Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase (affinty purified, goat antibody), Polyclonal

Product Overview

• Article Name: Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase (affinty purified, goat antibody), Polyclonal
• Biozol Catalog Number: AGR-AS09-577A
• Supplier Catalog Number: AS09-577A
• Alternative Catalog Number: AGR-AS09-577A
• Manufacturer: Agrisera
• Host: Goat
• Category: Antikörper
• Application: IF, WB
• Species Reactivity: A. thaliana, Plant
• Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Product Description

Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well house-keeping and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 26 | 31 kDa (Arabidopsis thaliana)
• NCBI: 826716
• UniProt: Q39258
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Form: Lyophilized
• Antibody Type: Polyclonal Antibody

Application Notes

• Application Dilute: 1: 600 (IF), 1 : 1000-1 : 3000 (WB)
• Application Notes: V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to i