Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
Clonality:
Monoclonal
Isotype:
IgG2a
Purity:
Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
Form:
Liquid
Antibody Type:
Monoclonal Antibody
Application Dilute:
1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
Application Notes:
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific.0.7µg/ml was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot.Antibody works paraffin-embedded sections.
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