Anti-Sml1 | Suppressor of Mec1 lethality, Polyclonal
Biozol Catalog Number:
AGR-AS10-847
Supplier Catalog Number:
AS10-847
Alternative Catalog Number:
AGR-AS10-847
Manufacturer:
Agrisera
Host:
Rabbit
Category:
Antikörper
Application:
WB
Species Reactivity:
Yeast
Immunogen:
KLH-conjugated synthetic peptide derived from known S.cerevisie Sml1 sequence. Gene ID: 854945
Sml1 is a ribonucleotide reductase inhibitor involved in regulation of dNTP production. It is regulated by Mec1p and Rad53p during DNA damage and S phase. Synonymes: Yml058w.
Cell preparation for western blot: cells were harvested by centrifugation (4000 x g , 6 min, 4 C), Supernatant was discarded and cells were resuspended in 500 µl cold TCA buffer (20 mM Tris, pH 8, 50 mM ammonium acetate, 2 mM EDTA, 1 tablet/10 ml of Comp
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