His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzy
Clonality:
Monoclonal
Isotype:
IgG2b
Purity:
Total IgG fraction. Protein A purified.
Form:
Liquid
Antibody Type:
Monoclonal Antibody
Application Dilute:
1 : 1000 (WB)
Application Notes:
Antibody is present in 10 mM PBS, pH 7.2His-tag (6,8,10xHis) needs to be properly exposed to allow detection. To prevent target protein folding, extraction should be performed with 6 to 8 M urea or using TCA-acetone precipitation method.
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