FtsH2 | FtsH2 positive control/quantitation standard
Biozol Catalog Number:
AGR-AS11-1789S
Supplier Catalog Number:
AS11-1789S
Alternative Catalog Number:
AGR-AS11-1789S
Manufacturer:
Agrisera
Category:
Sonstiges
Application:
WB
FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Loc
Molecular Weight:
75 kDa
Form:
Lyophilized
Antibody Type:
Secondary Antibody
Application Dilute:
Standard curve: 3 loads are recommended (0.5, 2 and 4µl).For most applications a sample load of 0.2µg of chlorophyll will give a FtsH2 signal in this range.Positive control: load per well: a 2 µl load is optimal for most chemiluminescent detection system
Application Notes:
Concentration: after adding 225 l of sterile milliQ water final concentration of the standard is 0.1 pmoles/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51mM, SERVA Blue G250 0.22 mM, Phenol Red 0
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