KLH-conjugated peptide, derived from Arabidopsis thaliana S6K1: UniProt: P42818, TAIR: AT3G08730 and S6K2: UniProt: Q39030, TAIR: AT3G08720. Due to high amino acid homology, chosen peptide is conserved in both proteins: S6K1 and S6K2.
Ribosomal S6 kinase 1/2 (S6K1/2) involved in TOR signaling pathway, in osmotic stress response. Activated by PDK1 and repressed during osmotic stress. Expressed in all tissues, especially during high metabolic activity in growing buds, root tips, leaf ma
Keeping the samples at 4 C all times is of crucial importance, Laucs buffer was used in example below since this is the one which was used routinely by this test laboratory for the work with kinases, Some unspecific bands can be seen, depending upon west
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