SBP | Sedoheptulose-1,7-bis phosphatase positive control/quantitation standard
Biozol Catalog Number:
AGR-AS15-2873S
Supplier Catalog Number:
AS15-2873S
Alternative Catalog Number:
AGR-AS15-2873S
Manufacturer:
Agrisera
Category:
Sonstiges
Application:
WB
SBPase (Sedoheptulose-1,7-bis phosphatase) is a chloroplast enzyme involved in the carbon reduction of the Calvin cycle, part of carbohydrate metabolism.Source of SBPase standard: SBPase standard source is Sphingomonas wittichii strain RW1, overexpressed
Molecular Weight:
27 kDa
Form:
Lyophilized
Antibody Type:
Secondary Antibody
Application Dilute:
Standard curve: 3 loads are recommended (0.5, 2 and 4µl).For most applications a sample load of 0.2 µg of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection systems
Application Notes:
Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Pheno
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