GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Clonality:
Polyclonal
Molecular Weight:
26 kDa
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Form:
Lyophilized
Antibody Type:
Polyclonal Antibody
Application Dilute:
1: 1: 1000 (ELISA), 1: 2000 (WB)
Application Notes:
Overnight incubation of this antibody with dilution 1:5 000 with 0,5 g of GST-tagged protein produced strong background but works fine with samples of total Escherichia coli lysate if GST-tagged protein is expressed in low amounts.
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