Anti-EMD, IgG1, Clone: [CL0203], Mouse, Monoclonal

Catalog Number: ATA-AMAB90562
Article Name: Anti-EMD, IgG1, Clone: [CL0203], Mouse, Monoclonal
Biozol Catalog Number: ATA-AMAB90562
Supplier Catalog Number: AMAb90562
Alternative Catalog Number: ATA-AMAB90562-100,ATA-AMAB90562-25
Manufacturer: Atlas Antibodies
Host: Mouse
Category: Antikörper
Application: ICC, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: LEMD5, STA
emerin
Anti-EMD
Clonality: Monoclonal
Concentration: 0.1
Clone Designation: [CL0203]
Isotype: IgG1
NCBI: 2010
UniProt: P50402
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Protein A purified
Sequence: ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSACQSITHY
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: EMD
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 2-10 µg/ml, IHC: 1:5000 - 1:10000, WB: 1 µg/ml
Immunofluorescence staining in HeLa cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in A431 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in MCF7 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in U2OS cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in U251 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunohistochemical staining of human skin shows strong nuclear membrane positivity in the epidermis cells.
Immunohistochemical staining of human prostate shows strong nuclear membrane immunoreactivityy in glandular cells.
Immunohistochemical staining of human placenta shows strong nuclear membrane positivity in the trophoblast.
Immunohistochemical staining of human testis shows nuclear membrane immunoreactivity.
Immunohistochemical staining of human kidney shows nuclear membrane positivity in renal tubules.
Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Lane 1: Marker [kDa]
Lane 2: RT-4
AMAb90562
AMAb90562
AMAb90562