Anti-EZR, IgG1, Clone: [CL2384], Mouse, Monoclonal

Catalog Number: ATA-AMAB90976
Article Name: Anti-EZR, IgG1, Clone: [CL2384], Mouse, Monoclonal
Biozol Catalog Number: ATA-AMAB90976
Supplier Catalog Number: AMAb90976
Alternative Catalog Number: ATA-AMAB90976-100,ATA-AMAB90976-25
Manufacturer: Atlas Antibodies
Host: Mouse
Category: Antikörper
Application: ICC, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: VIL2, Pan-Cancer
ezrin
Anti-EZR
Clonality: Monoclonal
Concentration: 1
Clone Designation: [CL2384]
Isotype: IgG1
NCBI: 7430
UniProt: P15311
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Protein A purified
Sequence: REEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLE
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: EZR
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 2-10 µg/ml, IHC: 1:5000 - 1:10000, WB: 1 µg/ml
Immunofluorescence staining in HeLa cell line with Anti-EZR monoclonal antibody, showing specific staining of plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in A431 cell line with Anti-EZR monoclonal antibody, showing specific staining of plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in MCF7 cell line with Anti-EZR monoclonal antibody, showing specific staining of plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in U2OS cell line with Anti-EZR monoclonal antibody, showing specific staining of plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in U251 cell line with Anti-EZR monoclonal antibody, showing specific staining of plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunohistochemistry analysis in human small intestine and skeletal muscle tissues using AMAb90976 antibody. Corresponding EZR RNA-seq data are presented for the same tissues.
Immunohistochemical staining of human small intestine shows strong positivity in apical membranes in glandular cells.
Immunohistochemical staining of human placenta shows strong positivity in apical membrane in trophoblastic cells.
Immunohistochemical staining of human tonsil shows strong membranous positivity in germinal center cells.
Immunohistochemical staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-EZR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Lane 1: Marker [kDa]
Lane 2: Human cell line U-251 MG
AMAb90976
AMAb90976
AMAb90976