Anti-HSP90B1, IgG2b, Clone: [CL2647], Mouse, Monoclonal

Catalog Number: ATA-AMAB91019
Article Name: Anti-HSP90B1, IgG2b, Clone: [CL2647], Mouse, Monoclonal
Biozol Catalog Number: ATA-AMAB91019
Supplier Catalog Number: AMAb91019
Alternative Catalog Number: ATA-AMAB91019-100,ATA-AMAB91019-25
Manufacturer: Atlas Antibodies
Host: Mouse
Category: Antikörper
Application: ICC, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: GP96, GRP94, TRA1, Pan-Cancer
heat shock protein 90kDa beta (Grp94), member 1
Anti-HSP90B1
Clonality: Monoclonal
Concentration: 1
Clone Designation: [CL2647]
Isotype: IgG2b
NCBI: 7184
UniProt: P14625
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Protein A purified
Sequence: SGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTA
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: HSP90B1
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 2-10 µg/ml, IHC: 1:5000 - 1:10000, WB: 1 µg/ml
Immunofluorescence staining in HeLa cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in A431 cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in MCF7 cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in U2OS cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence staining in U251 cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunohistochemical staining of human prostate shows strong cytoplasmic immunoreactivity in glandular cells.
Immunohistochemical staining of human cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human rectum shows strong cytoplasmic immunoreactivity in glandular and lymphoid cells.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Western blot analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line U-251
AMAb91019
AMAb91019
AMAb91019