Anti-BID, Rabbit, Polyclonal

Catalog Number: ATA-HPA000722
Article Name: Anti-BID, Rabbit, Polyclonal
Biozol Catalog Number: ATA-HPA000722
Supplier Catalog Number: HPA000722
Alternative Catalog Number: ATA-HPA000722-25,ATA-HPA000722-100
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ICC, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: Pan-Cancer
BH3 interacting domain death agonist
Anti-BID
Clonality: Polyclonal
Concentration: 0.3 mg/ml
Isotype: IgG
NCBI: 637
UniProt: P55957
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: VNNGSSLRDECITNLLVFGFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQLRNTSRSEEDRNRDLATALEQLLQAYPRDMEKEKTMLVLALLLAKKVASHTPSLLRDVFHTTVNFINQNLRTYVRSLAR
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: BID
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:1000 - 1:2500, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry analysis in human bone marrow and testis tissues using Anti-BID antibody. Corresponding BID RNA-seq data are presented for the same tissues.
Immunohistochemical staining of human bone marrow shows high expression.
Immunohistochemical staining of human testis shows low expression as expected.
Western blot analysis in human cell lines MCF-7 and HeLa using Anti-BID antibody. Corresponding BID RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18
Lane 2: Human cell line RT-4
Lane 3: Human cell line EFO-21
Lane 4: Human cell line A-431
Lane 5: Human liver tissue
Lane 6: Human tonsil tissue
HPA000722
HPA000722
HPA000722