Anti-G6PD, Rabbit, Polyclonal

Catalog Number: ATA-HPA000834
Article Name: Anti-G6PD, Rabbit, Polyclonal
Biozol Catalog Number: ATA-HPA000834
Supplier Catalog Number: HPA000834
Alternative Catalog Number: ATA-HPA000834-25,ATA-HPA000834-100
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ICC, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: G6PD1
glucose-6-phosphate dehydrogenase
Anti-G6PD
Clonality: Polyclonal
Concentration: 0.2 mg/ml
Isotype: IgG
NCBI: 2539
UniProt: P11413
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: FQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMLH
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: G6PD
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:2500 - 1:5000, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA000834 antibody. Corresponding G6PD RNA-seq data are presented for the same tissues.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in a subset of lymphoid cells.
Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in lymphoid cells.
Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in Kupffer cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
Western blot analysis in human cell lines A-549 and HEK293 using Anti-G6PD antibody. Corresponding G6PD RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Western blot analysis using Anti-G6PD antibody HPA000834 (A) shows similar pattern to independent antibody HPA000247 (B).
HPA000834
HPA000834
HPA000834