Anti-KRT18, Rabbit, Polyclonal

Product Overview

• Article Name: Anti-KRT18, Rabbit, Polyclonal
• Biozol Catalog Number: ATA-HPA001605
• Supplier Catalog Number: HPA001605
• Alternative Catalog Number: ATA-HPA001605-25,ATA-HPA001605-100
• Manufacturer: Atlas Antibodies
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
• Conjugation: Unconjugated
• Alternative Names: Pan-Cancer

Product Description

keratin 18

Additional Information

Anti-KRT18

Product Properties

• Clonality: Polyclonal
• Concentration: 0.05 mg/ml
• Isotype: IgG
• NCBI: 3875
• UniProt: P05783
• Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
• Purity: Affinity purified using the PrEST antigen as affinity ligand
• Sequence: IEESTTVVTTQSTEVGAAKAMLTELTRTVQFLEINLDSMRNLKASLENSLREVVARYALQMEQLNGILLHLESELAQTWAEGQCQAQEYQALLNIKVKLEAAIATYRRLLEDG
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
• Target: KRT18
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:50 - 1:200, WB: 0.04-0.4 µg/ml

Images

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Immunohistochemical staining of human kidney shows moderate cytoplasmic and membrane positivity in cells in tubules.

Immunohistochemical staining of human small intestine shows moderate cytoplasmic and membrane positivity in glandular cells.

Immunohistochemical staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.

Immunohistochemical staining of human placenta shows moderate cytoplasmic and membrane positivity in trophoblastic cells.

Western blot analysis in human cell lines MCF-7 and SK-MEL-30 using Anti-KRT18 antibody. Corresponding KRT18 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

HPA001605

HPA001605

HPA001605