Anti-VIM, Rabbit, Polyclonal
Catalog Number:
ATA-HPA001762
- Images (14)
| Article Name: | Anti-VIM, Rabbit, Polyclonal |
| Biozol Catalog Number: | ATA-HPA001762 |
| Supplier Catalog Number: | HPA001762 |
| Alternative Catalog Number: | ATA-HPA001762-25,ATA-HPA001762-100 |
| Manufacturer: | Atlas Antibodies |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ICC, IHC, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence |
| Conjugation: | Unconjugated |
| Alternative Names: | VIM |
| vimentin |
| Anti-VIM |
| Clonality: | Polyclonal |
| Concentration: | 0.2 mg/ml |
| Isotype: | IgG |
| NCBI: | 7431 |
| UniProt: | P08670 |
| Buffer: | 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. |
| Purity: | Affinity purified using the PrEST antigen as affinity ligand |
| Sequence: | FANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDSLARLDLERKVES |
| Storage: | Store at +4°C for short term storage. Long time storage is recommended at -20°C. |
| Target: | VIM |
| Antibody Type: | Monoclonal Antibody |
| Application Dilute: | ICC-IF: 0.25-2 µg/ml, IHC: 1:1000 - 1:2500, WB: 0.04-0.4 µg/ml |
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Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments. |
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Immunohistochemistry analysis in human ovary and skeletal muscle tissues using HPA001762 antibody. Corresponding VIM RNA-seq data are presented for the same tissues. |
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Immunohistochemical staining of human fallopian tube shows moderate membranous positivity in glandular cells. |
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Immunohistochemical staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells. |
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Immunohistochemical staining of human testis shows strong membranous positivity in cells in seminiferous ducts. |
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Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes. |
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Immunohistochemical staining of human ovary shows moderate cytoplasmic positivity in stromal cells. |
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Western blot analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH. |
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Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12. |
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HPA001762 |
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HPA001762 |
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HPA001762 |
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