Anti-SIX1, Rabbit, Polyclonal
Catalog Number:
ATA-HPA001893
- Images (14)
| Article Name: | Anti-SIX1, Rabbit, Polyclonal |
| Biozol Catalog Number: | ATA-HPA001893 |
| Supplier Catalog Number: | HPA001893 |
| Alternative Catalog Number: | ATA-HPA001893-25,ATA-HPA001893-100 |
| Manufacturer: | Atlas Antibodies |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ICC, IHC, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence |
| Conjugation: | Unconjugated |
| Alternative Names: | DFNA23 |
| SIX homeobox 1 |
| Anti-SIX1 |
| Clonality: | Polyclonal |
| Concentration: | 0.4 mg/ml |
| Isotype: | IgG |
| NCBI: | 6495 |
| UniProt: | Q15475 |
| Buffer: | 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. |
| Purity: | Affinity purified using the PrEST antigen as affinity ligand |
| Sequence: | CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS |
| Storage: | Store at +4°C for short term storage. Long time storage is recommended at -20°C. |
| Target: | SIX1 |
| Antibody Type: | Monoclonal Antibody |
| Application Dilute: | ICC-IF: 0.25-2 µg/ml, IHC: 1:500 - 1:1000, WB: 0.04-0.4 µg/ml |
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Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli. |
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Immunohistochemistry analysis in human skeletal muscle and liver tissues using HPA001893 antibody. Corresponding SIX1 RNA-seq data are presented for the same tissues. |
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Immunohistochemical staining of human salivary gland shows moderate to strong nuclear positivity in glandular cells. |
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Immunohistochemical staining of human breast cancer shows weak to moderate nuclear positivity in tumor cells. |
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Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected. |
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Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in striated muscle fibers. |
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Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SIX1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB. |
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Western blot analysis in mouse cell line NIH-3T3. |
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Western blot analysis in control (vector only transfected HEK293T lysate) and SIX1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401814). |
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HPA001893 |
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HPA001893 |
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HPA001893 |
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