Anti-ECD, Rabbit, Polyclonal

Catalog Number: ATA-HPA006465
Article Name: Anti-ECD, Rabbit, Polyclonal
Biozol Catalog Number: ATA-HPA006465
Supplier Catalog Number: HPA006465
Alternative Catalog Number: ATA-HPA006465-25,ATA-HPA006465-100
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ICC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: GCR2, hSGT1
ecdysoneless homolog (Drosophila)
Anti-ECD
Clonality: Polyclonal
Concentration: 0.1 mg/ml
Isotype: IgG
NCBI: 11319
UniProt: O95905
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: DEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKP
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: ECD
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:200 - 1:500, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemical staining of human colon shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemical staining of human endometrium shows weak to moderate nuclear positivity in stromal cells.
Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human duodenum shows moderate to strong nuclear positivity in glandular cells.
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
HPA006465
HPA006465