Anti-MCAM, Rabbit, Polyclonal

Product Overview

• Article Name: Anti-MCAM, Rabbit, Polyclonal
• Biozol Catalog Number: ATA-HPA008848
• Supplier Catalog Number: HPA008848
• Alternative Catalog Number: ATA-HPA008848-25,ATA-HPA008848-100
• Manufacturer: Atlas Antibodies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
• Conjugation: Unconjugated
• Alternative Names: CD146, MUC18, Pan-Cancer

Product Description

melanoma cell adhesion molecule

Additional Information

Anti-MCAM

Product Properties

• Clonality: Polyclonal
• Concentration: 0.3 mg/ml
• Isotype: IgG
• NCBI: 4162
• UniProt: P43121
• Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
• Purity: Affinity purified using the PrEST antigen as affinity ligand
• Sequence: LNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLE
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
• Target: MCAM
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Dilute: IHC: 1:200 - 1:500, WB: 0.04-0.4 µg/ml

Images

Immunohistochemistry analysis in human placenta and pancreas tissues using HPA008848 antibody. Corresponding MCAM RNA-seq data are presented for the same tissues.

Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in endothelial cells.

Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.

Immunohistochemical staining of human pancreas shows weak cytoplasmic positivity in endothelial cells.

Western blot analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-MCAM antibody. Corresponding MCAM RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Western blot analysis in human cell line WM-115.

HPA008848

HPA008848

HPA008848